Electron paramagnetic resonance spectrometer for three‐dimensionalinvivoimaging at very low frequency
作者:
M. Alecci,
S. Della Penna,
A. Sotgiu,
L. Testa,
I. Vannucci,
期刊:
Review of Scientific Instruments
(AIP Available online 1992)
卷期:
Volume 63,
issue 10
页码: 4263-4270
ISSN:0034-6748
年代: 1992
DOI:10.1063/1.1143723
出版商: AIP
数据来源: AIP
摘要:
The paper describes an electron paramagnetic resonance apparatus for spectroscopy and imaging at very low frequency (283 MHz). The bridge operates in a reflection cavity homodyne configuration and can be used on a very broad frequency range. The sample cavity is a one loop‐two gap resonator and accepts samples up to 5 cm in diameter and 10 cm long. These sample dimensions make the apparatus suitable for observing living samples such as rats. For the main field and gradients the apparatus uses a newly designed multipolar magnet that can provide the main field and gradients for 2D image reconstruction. The third gradient is obtained by coils mounted in the bore of the magnet. Tests on peroxilamine disulfonate samples have shown micromolar sensitivity. The minimum spatial resolution is limited by the sample linewidth and by the signal/noise. In living samples the toxicity of nitroxide radicals restricts the maximum label concentrations to values of the order of 10−4M, and the resolution seems to be limited by sensitivity to fractions of cm3.
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