Role of Arachidonic Acid or Its Metabolites in Growth-Hormone-Releasing Factor-Induced Release of Somatostatin from the Median Eminence
作者:
Cecilia Aguila,
Ljiljana Milenkovic,
Samuel M. McCann,
Gary D. Snyder,
期刊:
Neuroendocrinology
(Karger Available online 1990)
卷期:
Volume 52,
issue 3
页码: 238-242
ISSN:0028-3835
年代: 1990
DOI:10.1159/000125592
出版商: S. Karger AG
关键词: Arachidonic acid;SRIF;GRF;Median eminence;In vitro
数据来源: Karger
摘要:
The possible involvement of arachidonic acid (AA) release in growth-hormone-releasing factor (GRF)-induced somatostatin (SRIF) release from the median eminence (ME) of the hypothalamus was evaluated in adult male rats using an in vitro incubation system. The MEs were preincubated with [14C]-AA, then washed and incubated with vehicle or test agents, and the release of SRIF and [14C]-AA into the medium was measured. In the experiments designed only to determine SRIF release, the MEs were first preincubated for 30 min. The medium was then discarded and replaced with fresh buffer or test substances and incubated for 10, 20 and/or 30 min. GRF (10–10M) stimulated both AA and SRIF release significantly within 20 min, with maximum release occurring at 30 min. The stimulatory effect of GRF on AA release was coincident with the release of SRIF. A phospholipase A2 inhibitor (10–6M, quinacrine) completely abolished the stimulatory effect of GRF on both AA and SRIF release. The release of SRIF induced by GRF was also inhibited by both indomethacin (10–6M, a cyclooxygenase inhibitor) and metyrapone (10-6M, a cytochrome P-450 inhibitor). On the other hand, nordihydroguaiaretic acid (10–6M, a lipoxygenase inhibitor) had no effect on GRF-evoked SRIF release. The data presented here suggest that an important GRF-mediated event leading to SRIF secretion is an elevated release of AA from ME fragments in vitro. In conclusion, our data are suggestive that the stimulatory effect of GRF on SRIF release is due, in part, to the release and subsequent metabolism of AA to one or more meta
点击下载:
PDF
(947KB)
返 回