Prolonged normothermic perfusion of the isolated bovine eye: initial results
作者:
de CooFred A.M.,
ZonnenbergBernard A.,
TrapNico H.,
期刊:
Current Eye Research
(Taylor Available online 1993)
卷期:
Volume 12,
issue 4
页码: 293-301
ISSN:0271-3683
年代: 1993
DOI:10.3109/02713689308999453
出版商: Taylor&Francis
数据来源: Taylor
摘要:
This paper describes initial results of experiments with a prolonged normothermic arterial perfusion of isolated bovine eyes. The eyes were perfused using oxygenated serum-free MEM. With respect to utilization of oxygen and use of glucose, viability could be proven for 5 days. After addition of some essential serum substitutes, viability could be extended to 9 days, moreover, doubled glucose utilization and decreasing LDH activity could also be measured. To prolonge and improve viability, much attention should be paid to oxygenation and composition of the perfusate.
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