Measurement of small forces using an optical trap
作者:
Lucien P. Ghislain,
Neil A. Switz,
Watt W. Webb,
期刊:
Review of Scientific Instruments
(AIP Available online 1994)
卷期:
Volume 65,
issue 9
页码: 2762-2768
ISSN:0034-6748
年代: 1994
DOI:10.1063/1.1144613
出版商: AIP
数据来源: AIP
摘要:
A simple and sensitive method for detecting small forces applied to an optically trapped microsphere utilizes the transmitted beam of the trapping laser to monitor microsphere deflections within the potential well of the trap. The rms dynamic displacement detection sensitivity measured in the frequency range from 1 Hz to 10 kHz is ∼1 nm in the radial direction and ∼10 nm along thez(optic) axis. Radiation trapping force constants were calibrated against viscous drag on microspheres in the range between the Rayleigh (r<0.2&lgr;) and Mie (r≫&lgr;) size regimes. For a 1‐&mgr;m‐diam polystyrene sphere trapped with a 60 mW beam the rms spontaneous thermal motion limits the force sensitivity to better than 10−12N and lateral spatial resolution to ∼10 nm in a frequency range from 1 Hz to the viscous rolloff frequency ∼1 kHz. The measured maximum trapping efficiencies are compared with the theoretical predictions of the ray‐optics approximation.
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