Transformation studies ofActinidia chinensisPlanch.
作者:
LenaG. Fraser,
Jacqueline Kent,
CatherineF. Harvey,
期刊:
New Zealand Journal of Crop and Horticultural Science
(Taylor Available online 1995)
卷期:
Volume 23,
issue 4
页码: 407-413
ISSN:0114-0671
年代: 1995
DOI:10.1080/01140671.1995.9513917
出版商: Taylor & Francis Group
关键词: Actinidia chinensis;Actinidia deliciosa;kiwifruit;regeneration;transformation
数据来源: Taylor
摘要:
Transformed plants of four different genotypes ofActinidia chinensisPlanch, were recovered from experiments using theAgrobacterium tumefaciensstrains A281 and C58, both carrying the binary vector pKIWI105.A. chinensiswas compared to a related species A.deliciosa,in its amenability to transformation and regeneration procedures. During regenerationA. chinensisshoot initiation was more rapid and prolific thanA. deliciosa,althoughA. chinensiswas more sensitive to small changes in growth regulator concentration. Comparisons between the different genotypes ofA. chinensisshowed only minor differences in growth regulator preferences. The strains ofAgrobacterium tumefaciensthat were effective as gene transfer agents in A.chinensiswere the same as those which transformed A.deliciosa.Southern analysis and Polymerase Chain Reaction amplification of the transferred genes were used to confirm the transgenic nature of regenerated plantlets.
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