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Transformation studies ofActinidia chinensisPlanch.

 

作者: LenaG. Fraser,   Jacqueline Kent,   CatherineF. Harvey,  

 

期刊: New Zealand Journal of Crop and Horticultural Science  (Taylor Available online 1995)
卷期: Volume 23, issue 4  

页码: 407-413

 

ISSN:0114-0671

 

年代: 1995

 

DOI:10.1080/01140671.1995.9513917

 

出版商: Taylor & Francis Group

 

关键词: Actinidia chinensis;Actinidia deliciosa;kiwifruit;regeneration;transformation

 

数据来源: Taylor

 

摘要:

Transformed plants of four different genotypes ofActinidia chinensisPlanch, were recovered from experiments using theAgrobacterium tumefaciensstrains A281 and C58, both carrying the binary vector pKIWI105.A. chinensiswas compared to a related species A.deliciosa,in its amenability to transformation and regeneration procedures. During regenerationA. chinensisshoot initiation was more rapid and prolific thanA. deliciosa,althoughA. chinensiswas more sensitive to small changes in growth regulator concentration. Comparisons between the different genotypes ofA. chinensisshowed only minor differences in growth regulator preferences. The strains ofAgrobacterium tumefaciensthat were effective as gene transfer agents in A.chinensiswere the same as those which transformed A.deliciosa.Southern analysis and Polymerase Chain Reaction amplification of the transferred genes were used to confirm the transgenic nature of regenerated plantlets.

 

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