A test using marked flies was devised to determine if roadways or other open lanes through woodland are the major flight route used by femaleTabanusspp. (PrimarilyT. fusciocostatusandT. lineolaFab.) in long flights (≥ one km) in a south Louisiana bottomland hardwood forest. The usage of lane routes versus wooded routes was tested by comparing the number of marked tabanids that were recaptured at the end of each route. Statistical analysis revealed that 95% of these comparisons showed no significant difference (P>.05). These data and other considerations indicate that roadways are probably not used for the majority of long flights in South Louisiana forests.