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Enzyme Sensor for the Detection of Herbicides Inhibiting Acetolactate Synthase

 

作者: Atsushi Seki,   Florence Ortéga,   Jean-Louis Marty,  

 

期刊: Analytical Letters  （Taylor Available online 1996）
卷期: Volume 29, issue 8  

页码: 1259-1271

 

ISSN:0003-2719

 

年代: 1996

 

DOI:10.1080/00032719608001479

 

出版商: Taylor & Francis Group

 

关键词: acetolactate synthase;oxygenase activity;amperometric detection;sulfonylurea;imidazolinone;biosensor

 

数据来源: Taylor

 

摘要:

An enzyme sensor for the detection of sulfonylurea herbicides inhibiting acetolactate synthase II (ALS) was developed using an oxygen electrode. ALS, which has an oxygen consumption side reaction, was entrapped in the matrix of PVA-SbQ polymer, and the enzyme membrane was attached to the electrode. The inhibition of side oxygen reaction of ALS II is measured as decreased consumption of O2monitored by an oxygen electrode. Preliminary results show that 10−6M herbicide concentration can be determined by this method.

 

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