Microcalorimetric Determination of Serum Triglycerides and Cholesterol
作者:
Y.T. Das,
S.K. Chattopadhyay,
H.D. Brown,
期刊:
Analytical Letters
(Taylor Available online 1982)
卷期:
Volume 15,
issue 6
页码: 519-527
ISSN:0003-2719
年代: 1982
DOI:10.1080/00032718208064395
出版商: Taylor & Francis Group
关键词: cholesterol;cholesterol oxidase;glycerol;lipase;microcalorimetry;triglycerides
数据来源: Taylor
摘要:
Reaction heats measured in a microcalorimeter between varying amounts of human serum and 700 IU of lipase (EC 3.1.1.3) and those between serum and 8 U of cholesterol oxidase (EC 1.1.3.6) were significantly linear with the contents of triglycerides (r=1.00) and cholesterol (r=0.99), respectively. However, when sera from 13 human volunteers were subject to comparative study between the micro-calorimetric and spectrophotometric methods, the correlation became less appreciable (r=0.70 and r=0.71) presumably due to the individual variations in free glycerol and cholesterol ester-constituents that were deliberately omitted in the microcalorimetric method but were routinely included in the standard (multi-enzymatic) spectro-photometric clinical method. The contribution of interfering substances, if any, is not totally ruled out.
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