Applied genetics in the gram positive bacteriumstaphylococcus carnosus
作者:
F. Götz,
Mikrobielle Genetik,
期刊:
Food Biotechnology
(Taylor Available online 1990)
卷期:
Volume 4,
issue 1
页码: 505-513
ISSN:0890-5436
年代: 1990
DOI:10.1080/08905439009549763
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Staphylococcus carnosushas been used for a long time in the food industry as a starter culture. A host/vector system using this microorganism has been developed in order to investigate heterologous gene expression and to produce certain enzymes and hormones. The methods used for cloning are briefly described. TheStaphylococcus hyicuslipase gene has been studied in more detail. Gene expression and protein studies inS. carnosusrevealed that the lipase protein is composed of a signal peptide, a propeptide and the mature enzyme. The lipase gene was successfully used for gene fusion studies and to secrete heterologous proteins inS. carnosus.
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