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Horizontal two‐dimensional electrophoresis with immobilized pH gradients using PhastSystem

 

作者: Angelika Görg,   Wilhelm Postel,   Siegfried Günther,   Claudia Friedrich,  

 

期刊: ELECTROPHORESIS  （WILEY Available online 1988）
卷期: Volume 9, issue 1  

页码: 57-59

 

ISSN:0173-0835

 

年代: 1988

 

DOI:10.1002/elps.1150090111

 

出版商: Wiley Subscription Services, Inc., A Wiley Company

 

数据来源: WILEY

 

摘要:

AbstractProtocols for horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two‐dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first‐dimensional immobilized pH gradient gel strip prior to second‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis were evaluated. Silver stained two‐dimensional patterns were obtained w

 

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