Eight accessions of bermudagrass,Cynodon dactylon(L.) Pers., were evaluated for susceptibility to the bermudagrass mite,Eriophyes cynodoniensisSayed, under hydroponic culture in the greenhouse. Mite populations failed to become established on FB-119 during an 8-mo experiment. ‘Tifway’ and FB-141 became infested in only 44 and 50%, respectively, of the plants. No difference in infestation level resulted from varied N levels available to the plants. FB-119 has been observed and sampled in field plots adjacent to bermudagrass mite infestations, for ca. 6 yr with no infestation of this eriophyid mite. Susceptible cultivars, however, had continual infestations. The hydroponic culture technique was satisfactory for holding and screening the accessions for resistance.