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Micelle Stabilized Phosphorescent Immunoassay Based on Bispecific Antibodies Against Label and Antigen

 

作者: M.V. Demcheva,   E.Yu. Mantrova,   A.P. Savitsky,   O. Behrsing,   B. Micheel,   I. Hemmila,  

 

期刊: Analytical Letters  （Taylor Available online 1995）
卷期: Volume 28, issue 2  

页码: 249-258

 

ISSN:0003-2719

 

年代: 1995

 

DOI:10.1080/00032719508000319

 

出版商: Taylor & Francis Group

 

关键词: phosphorescence;Pd-coproporphyrin;hCG;hybrid hybridoma;bifunctional monoclonal antibody

 

数据来源: Taylor

 

摘要:

Hybrid hybridomas producing bispecific antibodies against α-subunit of hCG and Pd-coproporphyrin are obtained by using FACS. Conjugates of Pd-coproporphyrin with different proteins are tested for better sensitivity in micelle stabilized phosphorescent immunoassay. The role of steric factors for interaction of immobilized antibodies with antigens are discussed.

 

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