Capillary electrophoretic analysis of flavonoids
作者:
Ulrike Seitz,
Peter J. Oefner,
Suraphol Nathakarnkitkool,
Michael Popp,
Günther K. Bonn,
期刊:
ELECTROPHORESIS
(WILEY Available online 1992)
卷期:
Volume 13,
issue 1
页码: 35-38
ISSN:0173-0835
年代: 1992
DOI:10.1002/elps.1150130107
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractCombining the effects of electrophoresis and electroendosmosis, flavonoids were separated in less than ten minutes in a fused silica capillary tube with a borate buffer adjusted to pH 10. An increase in the concentration of borate from 0.1 to 0.2Mresulted in longer migration times due to a decrease in electroosmotic flow, but also in improved selectivity and higher resolution of flavonoids. The calibration curve of rutin showed a detection limit of 0.02 mg/mL and linearity over its pharmaceutical concentration range. Using an internal standard of known concentration, the content of rutin in a methanolic extract ofSambuci floscould be determined with a coefficient of variation as small as 3.8% by the molar ratio – peak area ratio metho
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