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Microanalytical ultracentrifuge

 

作者: Pavel E. Moroz,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1980)
卷期: Volume 51, issue 9  

页码: 1247-1252

 

ISSN:0034-6748

 

年代: 1980

 

DOI:10.1063/1.1136411

 

出版商: AIP

 

数据来源: AIP

 

摘要:

An ultracentrifuge based on the air turbine of a dental drill attains 400,000–600,000 rpm and a centrifugal force 2 to 3 million ×g. It is used for the examination of cell suspentions and homogenates and can be applied to macromolecular solutions. Microcuvettes with volumes ranging from less than 1 mm3to 0.001 mm3(suitable for single cells) are of disk and square capillary form and are made of polycarbonate or glass. A special microscope is used to obtain a stopped image of the rotating cuvette. A new method to preserve the centrifugal state of the contents is tested: deep freezing of a rotating cuvette for subsequent freeze‐drying and microscopical examination. Experiments on cells include centrifugal intercellular movement of nuclei and cytoplasmic particles, cell fusion, and homogenization.

 

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