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Estimation of protein synthesis rates using the flooding method and [15N] lysine

 

作者: K. Krawielitzki,   R. Schadereit,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  （Taylor Available online 1992）
卷期: Volume 28, issue 1  

页码: 8-12

 

ISSN:0021-1915

 

年代: 1992

 

DOI:10.1080/00211919208050795

 

出版商: Taylor & Francis Group

 

关键词: Animals;biochemical reaction kinetics;carbon 14;lysine;nitrogen 15;metabolism;organs;rats;tracer techniques

 

数据来源: Taylor

 

摘要:

After injection of a single dose of double labelled lysine (1 MBq L-[u-14C]lysine and 150 μmol L-[α15N]lysine (95 atom%15N)) to growing rats the fractional protein synthesis rates (FSR) of some organs were estimated by the “large dose-” or “flooding method”. Data obtained with both substances were compared and the following conclusions can be drawn:

 

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