To find out whether the composition of the subcutaneous adipose tissue of cats reflects the intake of polyunsaturated fatty acids, we performed a feeding trial. Six groups of kittens were fed on diets with variable combinations of corn, linseed, and fish oil. After 5 months, biopsies of subcutaneous adipose tissue were analysed for their contents of linoleic, α‐linolenic, eicosapentaenoic, and docosahexaenoic acid. The observed strong correlations between dietary and fat tissue polyunsaturated fatty acids indicate that the fatty acid composition of adipose tissue may be used as an index of the fatty acid composition of the diet. Thus, in epidemiological studies on the possible relationship between dietary fat type and feline disease the fatty acid composition of adipose tissue might be a useful measure.