Distribution of functional HIV-specific CD8 T lymphocytes between blood and secondary lymphoid organs after 8–18 months of antiretroviral therapy in acutely infected patients
作者:
Annette Oxenius,
Sabine Yerly,
Elbe Ramirez,
Rodney Phillips,
David Price,
Luc Perrin,
期刊:
AIDS
(OVID Available online 2001)
卷期:
Volume 15,
issue 13
页码: 1653-1656
ISSN:0269-9370
年代: 2001
出版商: OVID
关键词: Antiretroviral therapy;CD8 T lymphocytes;lymphoid organs;peripheral blood leukocytes
数据来源: OVID
摘要:
ObjectivesTo assess whether drug-induced suppression of the plasma viral load is associated with selective differential distribution of virus-specific CD8 T cells between the blood and secondary lymphoid organs.MethodsHIV-specific CD8 T lymphocyte responses were quantified in matched peripheral blood and lymph node samples from seven patients starting treatment shortly after infection, who received antiretroviral theray (ART) for a median of 14 months. Cells recovered from samples were subjected to IFN-γ ELISPOT analysis. A series of synthetic peptides corresponding to previously characterized cytotoxic T lymphocyte epitopes restricted by HLA I molecules present in each patient were used as antigens, together with appropriate positive and negative controls.ResultsHIV-specific CD8 T lymphocyte responses were found in six of the seven patients. The observed frequencies of HIV-specific CD8 T lymphocytes and the pattern of epitope recognition was identical within the two compartments. These results also confirm the observation that functional HIV-specific CD8 T cells are preserved on ART in most patients initiating treatment at the time of primary HIV-1 infection.ConclusionThis investigation demonstrated that patterns of antigenic immunodominance as well as frequencies of HIV-specific CD8 T lymphocytes are similar in blood and lymphoid tissue compartments in HIV-infected individuals. These findings support current approaches to the identification of HIV-specific CD8 T lymphocyte reactivity based on leukocytes isolated from blood even in patients with ART-induced suppression of viral load.
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