Secretion of Transforming Growth Factor Alpha and Expression of its Receptor in Human Mammary Cell Lines
作者:
McAndrewJoanne,
FernigDavid G.,
RudlandPhilip S.,
SmithJohn A.,
期刊:
Growth Factors
(Taylor Available online 1994)
卷期:
Volume 10,
issue 4
页码: 281-287
ISSN:0897-7194
年代: 1994
DOI:10.3109/08977199409010994
出版商: Taylor&Francis
关键词: epidermal growth factor receptor;transforming growth factor alpha;mammary gland
数据来源: Taylor
摘要:
AbstractThe secretion of transforming growth factor alpha (TGFa) and the expression of cell-surface receptors for epidermal growth factor (EGF) were measured in a series of human mammary cell lines. The amount of TGFa secreted by the cells did not correlate with the phenotype of the cells (epithelial or myoepithelial), the mechanism of immortalization of the cells (SV40 or spontaneous) or the source of the cells (normal mammary gland, benign hyperplastic lesion, malignant tumour). The level of expression of cell-surface receptors for EGF was markedly increased as a consequence of SV40-immortalization of mammary cells, but otherwise did not correlate with the phenotype of the cells or the source of the cells. Much of the increase was accounted for by the appearance of a large number of low-affinity receptors for EGF in the SV40-immortalized cells. It is suggested that one of the mechanisms whereby SV40-immortalization suppresses the senescence of primary cultures of human mammary epithelial cells involves increasing the level of expression of receptors for EGF. In contrast the level of secretion of TGFa by cells in culture is probably a consequence of the mechanisms of adaptation of each cell line to culture conditions, and does not reflect the level of secretion of TGFa by cells in vivo.
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