Glomerular Epithelial Cell Endocytosis in Puromycin-Induced Glomerulopathy
作者:
Yiming Wang,
Paul S. Bass,
Beryl Evans,
Hywel Thomas,
David R. Davies,
期刊:
Nephron
(Karger Available online 1992)
卷期:
Volume 62,
issue 1
页码: 84-89
ISSN:1660-8151
年代: 1992
DOI:10.1159/000187001
出版商: S. Karger AG
关键词: Endocytosis;Epithelium;Glomerulopathy;Proteinuria;Horseradish peroxidase;Cation
数据来源: Karger
摘要:
Glomerulopathy and nephrotic syndrome were induced in rats by intravenous puromycin aminonucleoside. Ten days after the injection of puromycin, the animals have developed heavy proteinuria. During this phase, glomerular epithelial cell endocytosis was studied by injecting a conjugate of horseradish peroxidase and poly-L-lysine. This conjugate has been shown to be endocytosed by glomerular epithelial cells. The rats were serially sacrificed from 1 min to 24 h after this injection. Peroxidase was localised cytochemically and observed at light and electron microscopy. The early events of endocytosis in glomerulopathy (namely the binding to the plasma membrane, the membrane invagination and the formation of the early vesicles) were qualitatively similar to those in the normal. The later events (the fusion of the vesicles and their movement within the cells) were inhibited. The results show that puromycin aminonucleoside damages epithelial cell endocytotic activity and affects the later processing of the conjugate within the cells.
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