Renal effects of acute renal denervation (DNX) were studied in anaesthetized rats. In a first series, whole kidney clearance measurements were made 120 and 240 min after unilateral DNX. At 240 min, urine production was 3.59±0.87 μL min‐1in control kidneys and 7.74±1.97 μL min‐1in denervated kidneys. The corresponding values for sodium excretion were 0.56±0.17 and 1.41±0.34 μmol min‐1, potassium excretion 0.48±0.08 and 0.97±0.37 μmol min‐1and glomerular filtration rate (GFR) 0.83±0.08 and 1.05±0.16 mL min‐1, respectively. In a second series, tubuloglomerular feedback (TGF) characteristics were determined with the stop‐flow pressure (Psf) technique. With increasing time, the sensitivity of the TGF mechanism diminished in denervated rats, as indicated by an increased turning point (TP). TP was significantly increased 2 h after DNX from 19.1±1.13 in control to 25.9±1.10 nL min‐1. TP was further increased 4 h after DNX to 37.3±3.12 nL min‐1. However, the maximal TGF response to increased flow in the late proximal tubule was not altered. But, Pstwas significantly higher in DNX rats than in the controls (47.4±1.01 vs. 43.0±1.53 mmHg) in spite of a lower blood pressure (107±2.9 vs. 119±2.2 mmHg). We conclude that intact renal nerves are essential for the setting of the TGF sensitiv