QUANTITATION OF A NEW MACROLIDE IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRIC DETECTION: COMPARISON WITH ELECTROCHEMICAL DETECTION
作者:
D.J. Daszkowski,
V.S. Ong,
S. Menacherry,
T. El-Shourbagy,
S.L. Carroll,
K.C. Marsh,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 5
页码: 641-657
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101687
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A method involving high performance liquid chromatography with tandem mass spectrometric detection was developed to determine the concentration of a new macrolide derivative, ABT-229, in human plasma. The analyte was extracted using liquid-liquid extraction with a mixture of ethyl acetate and hexane at basic pH. Detection was highly specific for the analyte of interest, as evidenced by the lack of interference in the chromatograms of drug-free plasma extracts. The lower limit of quantitation was 0.10 ng/mL based on 1 mL of extracted plasma. The linear calibration range of the procedure extended from 0.10 to 199 ng/mL. The method proved reliable and rugged; within-run and day-to-day relative standard deviations were less than 10%. The stability of ABT-229 under frozen storage, room temperature, and freeze/thaw conditions was found to be excellent. Plasma concentrations determined by the method were highly comparable with data obtained by electrochemical detection.
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