The concentration of protein solutions by normal freezing
作者:
Jan‐Christer Janson,
Bo Ersson,
Jerker Porath,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1974)
卷期:
Volume 16,
issue 1
页码: 21-39
ISSN:0006-3592
年代: 1974
DOI:10.1002/bit.260160104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractAn apparatus is described which is designed for preparative freeze concentration experiments by the technique of normal freezing. It has a capacity of approximately 22 liters distributed over twelve vessels. The influence of various geometrical and chemical parameters such as stirring speed, crystallization rate, and sample composition on the normal freezing of protein solutions are discussed. For dilute protein solutions (<0.1%) the concentration factor generally was 8‐ to 10‐fold with recoveries of 90–100 percent. With higher protein concentrations and at ionic strengths higher than approximately 0.05, the recovery was decreased. No loss of activity was detected when concentrating enzyme solu
点击下载:
PDF
(764KB)
返 回