The effect of transforming growth factor-alpha (TGF$aL) on rabbit and primate lens epithelial cellsin vitro
作者:
WickströmKerstin,
MadsenKjell,
期刊:
Current Eye Research
(Taylor Available online 1993)
卷期:
Volume 12,
issue 12
页码: 1123-1128
ISSN:0271-3683
年代: 1993
DOI:10.3109/02713689309033510
出版商: Taylor&Francis
数据来源: Taylor
摘要:
TGF$aL, a member of the epidermal growth factor family stimulates proliferation of various cells. In the search for molecules that regulate cell division in the lens epithelium, the effect of numerous growth factors including EGF and FGF has been determined. In this study, the effect of TGF$aL on lens epithelial cells (LEC) from rabbit and monkey was investigated and compared to FGF and EGF. Since TGF$aL is suggested to be a factor that is regulated by auto and/or paracrine mechanisms, an immunohistochemical visualisation of the factor in the rabbit lensin situwas performed.TGF$aL stimulates LEC in a dose-dependent manner with a maximal threefold increase in cellular proliferation at 100 ng/ml. At a concentration above 10 ng/ml the cells lost their epithelial morphology and became irregular and star-shaped. Since TGF$aL is a potent LEC mitogen and has shown to increase in aqueous humour after trauma to the eye, this factor might be one of the molecules that regulate normal cell division in the ocular lens as well as LEC growth after cataract surgery.
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