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Staining Plant Cells with Silver. I. The Salt-Nylon Technique

 

作者: StackStephen,   HerickhoffLisa,   ShermanJamie,   AndersonLorinda,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1991）
卷期: Volume 66, issue 2  

页码: 69-78

 

ISSN:1052-0295

 

年代: 1991

 

DOI:10.3109/10520299109110553

 

出版商: Taylor&Francis

 

关键词: silver staining;plant cells;synaptonemal complex;nucleolus;nucleolus organizer region;kinetochore;chromosome core

 

数据来源: Taylor

 

摘要:

A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.

 

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