Molecular Epidemiology of Vertical Human Immunodeficiency Virus Type 1 Transmission in GreeceEvidence of Non-B Subtypes
作者:
Dimitrios Paraskevis,
Emmanuil Magiorkinis,
Maria Theodoridou,
Glykeria Mostrou,
Vassiliki Papaevangelou,
Vassilios Kiosses,
Angelos Hatzakis,
Nikolaos Matsaniotis,
期刊:
Journal of Human Virology
(OVID Available online 1999)
卷期:
Volume 2,
issue 6
页码: 339-343
ISSN:1090-9508
年代: 1999
出版商: OVID
关键词: subtypes;HIV-1;vertical transmission.
数据来源: OVID
摘要:
Objectives:To investigate the subtype classification of the circulating virus strains among human immunodeficiency virus type 1 (HIV-1)-infected children in Greece.Study Design/Methods:Since the beginning of the acquired immunodeficiency syndrome (AIDS) epidemic in Greece in 1982, 23 children have been reported to be vertically infected with HIV-1. Blood samples were available for 19 of these children, and the C2-C4envregion was successfully amplified by nested polymerase chain reaction (PCR) for 16 subjects. HIV-1 subtype was established by the heteroduplex mobility assay (HMA) in 16 subjects and confirmed by DNA sequencing and phylogenetic analysis in 8 subjects.Results:Most subjects (9; 56%) fell into subtype B. However, a substantial proportion (44%) were classified as subtypes A (3; 19%), C (1; 6%), D (1; 6%), and I (2; 12%). According to epidemiologic information, 5 of 7 children infected with non-B HIV-1 subtypes were born to Greek parents.Conclusion:These findings clearly suggest that non-B strains have been introduced into Greece, providing evidence that HIV epidemic in this country will probably change profile over time. In addition, subtype I was identified in 2 HIV-1-infected children, both of whom were born to Greek parents.Journal of Human Virology 1999;2:339-343 © Lippincott Williams & Wilkins, Inc.
点击下载:
PDF
(3116KB)
返 回