ABSTRACTA survey of protozoa polluting bottled mineral water in Mexico was carried out using samples obtained from the three best‐selling brands of bottled mineral water in the country. The organisms were concentrated through filtration procedures and subsequently cultured in sterile media. The cultures were observed over four weeks, with identification to the level of genus and species. Most commonly found were the amoebaeNaegleria gruberi, Acanthamoeba astronyxis, andVahlkampfia vahlkampfi(trophic as well as cystic stages) plus one flagellate,Bodomorpha minima. No ciliates were detected. The public health importance of the findings is obvious, since some strains ofNaegleriaandAcanthamoebahave the potential to cause human disease that may lead to deat