Fibroblast Growth Factor-stimulated Phosphorylation of a Lipocortin I-like Protein is S-Phase Cell Cycle Specific in Human Vascular Endothelial Cells
作者:
PatteChristine,
BlanquetPierre R.,
期刊:
Growth Factors
(Taylor Available online 1992)
卷期:
Volume 7,
issue 2
页码: 107-116
ISSN:0897-7194
年代: 1992
DOI:10.3109/08977199209046400
出版商: Taylor&Francis
关键词: bFGF;phosphorylation;lipocortin;human endothelial cells;internalization
数据来源: Taylor
摘要:
AbstractWe examined whether the phosphorylation of a 34 kDa lipocortin I-like protein may be associated with internalization process of fibroblast growth factor (FGF) in human umbilical vein endothelial (HUVE) cells. We show that: 1) exposure of synchronized HUVE cells to basic FGF for an appreciable time lag (≥30 min) at 37°C and subsequent phosphorylation at 37°C are required to obtain an increased32P-labelling of a 34 kDa substrate; 2) this FGF-stimulated phosphorylation occurs in S phase but not G1 phase of the growth cycle; 3) the 34 kDa substrate appears to be phosphorylated on tyrosine residues; 4) a major fraction of the 34 kDa32P-labelled substrate is immunoprecipitated with an antibody that has been raised against human lipocortin/annexin of type I. It is suggested that internalized FGF-receptor/kinase complexes might be primarily responsible for the phosphorylation of the 34 kDa lipocortin I-related protein in S phase HUVE cells.
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