A new HPLC method to determine glutathione-protein mixed disulfide
作者:
SpectorAbraham,
MingGuo,
Ruey C.Ruey,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 1
页码: 47-51
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688608995165
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A sensitive method for measuring glutathione-protein mixed disulfides is described. The method is based on cleavage of the protein disulfides with performic acid followed by reaction with dinitrofluorobenzene and HPLC analysis with a Bondapak-anine column. Samples containing 0.1 nmoles or more of glutathione-protein mixed disulfide can be detected. The method has been used to demonstrate (a) the presence of low levels of glutathione mixed disulfide in gamma crystallin isolated from bovine lenses, (b) a dramatic increase in such mixed disulfides after exposure of denatured gamma crystallin to O2in the presence of glutathione, and (c) the formation of glutathione-protein mixed disulfide in lens epithelial cells exposed to 0.6 mM H2-O2- for one hour.
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