Human enteric viruses are believed responsible for numerous disease outbreaks caused by contaminated groundwater, but little is known about the survival of these organisms in groundwater. This study compared the ability of several viruses and bacteria to survive in groundwater. The organisms were contained in individual survival chambers and exposed to a continuous flow of groundwater from a 275 ft deep domestic well. The survival of the test organisms ranked in order of decreasing decay rates were coliphage f2, rotavirus SA‐11,Escherichia coli, echovirus‐1, fecal streptococcus, poliovirus‐1, and coxsackievirus B3. Low numbers of bacteria and viruses survived for the 24 day duration of the experiment. These results indicate that enteric viruses are capable of surviving for extended periods in groundwater.