A New Technique Facilitating Studies of Scant Cell Specimens
作者:
DuarteLeeann,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 4
页码: 200-202
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109969
出版商: Taylor&Francis
关键词: scant cells;cell buttons;plastic embedding
数据来源: Taylor
摘要:
A simple method is described by which multiple cytological and cytochemical studies can be done on a clinical sample that contains relatively few cells. The cells are concentrated by centrifugation. The cell pellet is fixed, frozen and embedded in plastic. Thin (2-μm) sections are cut from the plastic. Thus, each cell may appear in several sections and many slides can be made from a single specimen. The advantages of this method over cytospins and Millipore filter preparations of cell suspensions are optimal utilization of all cells, excellent morphological and immunological preservation and ease and reproducibility of this technique.
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