Etude in vitro de l'activité lipasique de souches bactériennes isolées de cæcum de lapins sur la trioléine marquée au14C
作者:
C. Bacques,
Nguyen Thi Cuc,
M. Serusclat,
A. Dorier,
J.P. Perret,
期刊:
Annals of Nutrition and Metabolism
(Karger Available online 1983)
卷期:
Volume 27,
issue 2
页码: 145-152
ISSN:0250-6807
年代: 1983
DOI:10.1159/000176634
出版商: S. Karger AG
关键词: Rabbit;Triolein;Lipase;Caecum;Bacteria
数据来源: Karger
摘要:
The lipase activity of bacterial species isolated from the caecum of conventional rabbits was studied by means of two groups of tests: (1) bacteriologic isolation and an identification of nine aerobic and anaerobic clones and (2) incubation of each of these clones in the appropriate medium in the presence of labelled triolein (oleyl 1–14C), a biochemical analysis of the radioactivity of the fractions obtained. The main results can be expressed in the following way: maximum hydrolysis of glycerides is achieved by two species: Bacillus lichenifor-mis and Clostridium sporogenes. The reaction is proportional to the incubation time for incubations of less than 8 h. The release of fatty acids is concomitant to the synthesis of phospholipids and of fatty acid methyl esters.
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