Enzyme immunoassay of histamine in foods
作者:
Pavel Rauch,
Pavel Rychetsky,
Igor Hochel,
Radovan Bilek,
Jean‐Luc Guesdon,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1992)
卷期:
Volume 4,
issue 2
页码: 67-72
ISSN:0954-0105
年代: 1992
DOI:10.1080/09540109209354754
出版商: Taylor & Francis Group
关键词: foodstuffs;histamine;immunoassay
数据来源: Taylor
摘要:
A competitive enzyme immunoassay of histamine in foodstuffs has been developed using a monoclonal antibody against a histamine‐benzoquinone adduct. In this assay, histamine present in food samples was treated with 1,4‐benzoquinone to form histamine‐benzoquinone by a simple and quick reaction and a histamine‐benzoquinone‐horse‐radish peroxidase conjugate was used as the labelled hapten. The apparent association constant (Ka) of the antibody used was 3.6 ×106l/mol and Gibbs’ energy of the immune complex formation has been estimated to find the optimal incubation time of the assay. The method enabled determination of histamine in fish, cheese, wine and beer at a concentration as low as 7 ng/ml with an accuracy of ± 15%. The recovery of the immunoassay was 88.9–114%. Cross‐reactivities of histidine, tyramine, tryptamine and its derivatives were lower than 0.001% and did not affect the assay.
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