Rabbit Heart Fatty Acid-Binding Protein Isolation, Characterization, and Application of a Monoclonal Antibody
作者:
Anne Knowlton,
Robert Burner,
Peter Brecher,
期刊:
Circulation Research
(OVID Available online 1989)
卷期:
Volume 65,
issue 4
页码: 981-988
ISSN:0009-7330
年代: 1989
出版商: OVID
数据来源: OVID
摘要:
A fatty acid-binding protein (FABP) was purified from rabbit heart and characterized with respect to size, isoelectric point, and tissue distribution. This protein was found in red muscle, diaphragm, and aorta, as well as in the heart. Amino acid composition of rabbit heart FABP differed only slightly from the human and rat proteins. Rabbit heart FABP was shown to bind two molecules of fatty acid. A monoclonal antibody was developed and used to demonstrate the feasibility of a one-step purification with affinity chromatography. Cross-reactivity was found between the human protein and the rabbit antibody, and an immunoassay was developed to human heart FABP. Levels of human heart FABP in the plasma of patients with acute myocardial infarction were significantly elevated (83 ± 9 μg/ml) compared with patients with pulmonary edema (52 ± 7 μg/ml) and normal volunteers (28 ± 5 μg/ml;p< O.OS, mean ± SEM).
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