Establishment of conditions for the transformation of nonaxenicDictyosteliumstrains
作者:
Malgorzata M. Lloyd,
Adriano Ceccarelli,
Jeffrey G. Williams,
期刊:
Developmental Genetics
(WILEY Available online 1990)
卷期:
Volume 11,
issue 5‐6
页码: 391-395
ISSN:0192-253X
年代: 1990
DOI:10.1002/dvg.1020110511
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Lipofectin;slime mould;transformation
数据来源: WILEY
摘要:
AbstractWe have studied the transient expression, inDictyosteliumcells growing on a bacterial food source, of a construct containing the coding region of the firefly luciferase gene inserted downstream of aDictyosteliumactin promoter. The fusion gene is not detectably expressed when DNA is introduced by calcium phosphate precipitation or by electroporation, but it is expressed when introduced using cationic liposomes (lipofectin). Using this latter procedure, we are able to transform cells with a G418 resistance vector and select stable, drug‐resistant transformants at a relatively low, but workable, efficiency. This technique will allow molecular genetics to be applied to the many important nonaxenicDictyosteliumstrain
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