&NA;Endothelial permeability (Pac) to carboxyfluorescein and Na+/K+ATPase pump site density were determined in human corneas following storage for 4 or 7 days at 4°C in either modified McCarey‐Kaufman (mMK) or K‐Sol media. Following 4 days of storage, Pac values for mMK‐and K‐Sol‐preserved corneas were not significantly different from those of their prestorage mates. After 7 days of storage, however, corneas stored in K‐Sol media showed a significant increase in Pac compared to their prestorage mates, whereas the mMK‐stored corneas showed no change in Pac. Na+/K+ATPase pump site density determined using [3H]ouabain was similar to a control group in the K‐Sol‐stored tissue but higher in the mMK‐stored tissue following 7 days of storage. These studies suggest that mMK medium maintains endothelial barrier function and Na+/K+ATPase pump site density at least as well as K‐Sol medium through 7 days of corneal storage.