A Mixture of Paraphenylenediamine and Imidazole: Its Effect on the Extraction of Lipid Droplets during Electron Microscopy Staining
作者:
NakaoIezo,
OkadaHiyoshi,
SenzakiHideto,
NishimuraRieko,
FujitaNaoki,
ShikataNobuaki,
MoriiSotokichi,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 4
页码: 219-223
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110069
出版商: Taylor&Francis
关键词: p-phenylenediamine;imidazole;lipid droplet;electron microscopy staining;lipid extraction
数据来源: Taylor
摘要:
To prevent extraction of lipids during a double staining procedure for electron microscopy, the tissue slices, double fixed with glutaraldehyde and osmium tetroxide to preserve microvesicular lipid droplets in the cytoplasm, were immersed for 2 hr in veronal buffer (pH 9.0) containing 0.5% p-phenylenediamine and 0.5% imidazole immediately after postfixation. The stained sections of the immersed tissue slice showed blackened, well circumscribed lipid droplets similar to those in corresponding unstained sections. Moreover, highly contrasting features of the cellular architecture could be visualized with the double stained, as well as routinely prepared sections.
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