Superoxide Dismutase Biosensors for Superoxide Radical Analysis
作者:
Luigi Campanella,
Gabriele Favero,
Mauro Tomassetti,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 13
页码: 2559-2581
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542988
出版商: Taylor & Francis Group
关键词: biosensors;superoxide radical;analysis;scavengers
数据来源: Taylor
摘要:
Some basic work has been performed on the development and optimisation of superoxide dismutase (SOD) biosensors for superoxide radical analysis. Initially we studied the possibility of obtaining a SOD biosensor using the Clark electrode as indicating sensor. However, the best results were obtained using as indicator a classical amperometric electrode for H2O2. In both cases the superoxide radical was generatedin situusing the xanthine/xanthine oxidase (XOD) enzyme system, while the SOD was immobilised in kappa-carrageenan gel. The first application was realised by studying the effectsin vitroon the superoxide radical of some molecules commonly accepted as radical scavengers.
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