Effects of Retinoic Acid and Bromodeoxyuridine on Human Melanoma-Associated Antigen Expression in Small Cell Lung Carcinoma Cells
作者:
V. Feyles,
L.K.J. Sikora,
R.C. McGarry,
L.M. Jerry,
期刊:
Oncology
(Karger Available online 1991)
卷期:
Volume 48,
issue 1
页码: 58-64
ISSN:0030-2414
年代: 1991
DOI:10.1159/000226896
出版商: S. Karger AG
关键词: Human melanoma-associated antigens;Small cell lung carcinoma;Bromodeoxyuridine;Substrate adhesion
数据来源: Karger
摘要:
The dispersed neuroendocrine system includes cells with different embryological derivations, sharing a common neuroendocrine (NE) program, as indicated by the expression of NE markers, some of which are shared antigenic determinants. We report here that the small cell lung carcinoma cells NC1-H69 express the two human melanoma-associated antigens (HMAA) NGA/LS62 an LSI09. Incubation of NCI-H69 cells with maturational inducers, such as retinoic acid and bromodeoxyuridine (BrdU), upregulated the expression of both HMAA. Exposure to BrdU for 4 weeks induced the appearance of a different phenotype in subpopulations of NCI-H69 cells, which became epithelioid, substrate-adherent, grew in monolayer and continued to express NE-associated antigens in variable amount. The shift in phenotype was not reversible after BrdU withdrawal and was maintained for at least 6 months in continuous culture. The substrate adhesion of NCI-H69 cells was paralleled by a change in NGA glycosylation pattern, thus suggesting a possible functional role for NGA in cell substrate adhesion/recognition.
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