T-Tropic Sequence of the V3 Loop Is Critical for HIV-1 Infection of CXCR4-Positive Colonic HT-29 Epithelial Cells
作者:
J. Trujillo,
Nathalie Goletiani,
Irene Bosch,
Colleen Kendrick,
Rick Rogers,
Elaine Trujillo,
Max Essex,
Joseph Brain,
期刊:
JAIDS Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 2000)
卷期:
Volume 25,
issue 1
页码: 1-10
ISSN:1525-4135
年代: 2000
出版商: OVID
关键词: HIV-1 V3;CXCR4;Colonic cells
数据来源: OVID
摘要:
Some colonic and neuronal cells which are CD4−but galactosyl ceramide-positive are susceptible to infection with HIV-1. We have previously shown that the T-cell tropic V3 loop of HIV-1 gp120 serves as a primary viral determinant for infectivity of CD4−neuronal cells. However, the nature of the V3 loop of HIV-1 needed for infection and the V3 loop's interaction with coreceptors on colonic epithelial cells have not been fully analyzed. By using HIV-1 molecular clones, we show that the T-cell tropic V3 domain is critical for HIV-1 infection of colonic HT-29 epithelial cells. Because T-cell tropic HIV-1 can use CXCR4 as a coreceptor in T cells, we set out to determine the role of CXCR4 during infection of HT-29 cells. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunostaining, we show that these epithelial cells of colonic origin express the chemokine receptor CXCR4. Importantly, antibody against CXCR4 or a neutralizing antibody against HIV-1 gp120 V3 loop blocks T-cell tropic HIV-1 entry into HT-29 cells. These data indicate that the V3 loop of HIV-1 and the chemokine receptor CXCR4 are both critical for HIV-1 infection of colonic HT-29 epithelial cells. An HIV-1 T-tropic virus may be responsible for the infection of human colonic epithelial cells in vivo.
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