Evaluation of an improved rapid coagglutination method for the serological grouping of beta-hemolytic streptococci
作者:
Daniel V. Lim,
Rosella D. Smith,
Susan Day,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1979)
卷期:
Volume 25,
issue 1
页码: 40-43
ISSN:0008-4166
年代: 1979
DOI:10.1139/m79-006
出版商: NRC Research Press
数据来源: NRC
摘要:
Grouping of beta-hemolytic streptococci was performed with the Phadebact® Streptococcus Test, a coagglutination method, and the results compared with serological grouping by the standard Lancefield precipitin method. Of 171 clinical specimens examined, 169 (98.8%) were grouped correctly by the Phadebact® Test after 24 h of continuous growth in Todd–Hewitt broth, In a parallel study, 96.9% of specimens that grew after only 4 h of incubation in broth were grouped correctly by the coagglutination method. In both studies, the accuracy of the coagglutination test was increased significantly by elimination of multiple-agglutination reactions through centrifugation of cultures and utilization of the supernatant fluid in the Phadebact® Test.
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