Medium and long term storage ofAnigozanthos manglesii(D. Don) pollen
作者:
N. Sukhvibul,
J. A. Considine,
期刊:
New Zealand Journal of Crop and Horticultural Science
(Taylor Available online 1993)
卷期:
Volume 21,
issue 4
页码: 343-347
ISSN:0114-0671
年代: 1993
DOI:10.1080/01140671.1993.9513792
出版商: Taylor & Francis Group
关键词: kangaroo paw;Anigozanthos manglesii;low temperature storage;dehydration;germination media;fluorescein diacetate
数据来源: Taylor
摘要:
The viability of kangaroo paw pollen (Anigozanthos manglesii) was estimated by germination in vitro and by fluorescence and the more reliable method was then used to assess the effect of storage conditions on pollen longevity. Pollen germination was more accurate than staining for pollen of low and intermediate viability levels. Effective storage could be achieved in a number of ways depending on the duration required. Dehydrated pollen retained viability longer in storage than did hydrated pollen. Pollen from intact anthers could be stored for up to 5 weeks at room temperature with 50% or better germination provided it was stored on silica gel. Pollen isolated from anthers and stored at 2°C had reduced viability in comparison with that stored at room temperature or at ‐80°C. Pollen stored at ‐196°C lost only 5% viability after 20 weeks and presumably this would be the most suitable method for long term storage for breeding or conservation purposes.
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