Direct regulation of GnRH transcription by CRF‐like peptides in an immortalized neuronal cell line
作者:
D Tellam,
M Perone,
I Dunn,
S Radovick,
J Brennand,
J Rivier,
M Castro,
D Lovejoy,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 14
页码: 3135-3140
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Reproduction;Sauvagine;Stress;Transcription;Urotensin-I
数据来源: OVID
摘要:
THE existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (−611 bp) or chicken (−3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRFR1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF-like peptides.
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