Monoclonal Antibodies to Human Glomerular Cells: A Marker for Glomerular Epithelial Cells
作者:
Wayne W. Hancock,
Robert C. Atkins,
期刊:
Nephron
(Karger Available online 1983)
卷期:
Volume 33,
issue 2
页码: 83-90
ISSN:1660-8151
年代: 1983
DOI:10.1159/000182918
出版商: S. Karger AG
关键词: Monoclonal antibody;Cell surface antigens;Glomerular epithelial cells;Glomerular culture;Immunoperoxidase technique
数据来源: Karger
摘要:
A monoclonal antibody, PHM 5, directed against human glomerular epithelial cells, was prepared after immunisation of a mouse with isolated human glomeruli and fusion of spleen cells with a mouse myeloma. Binding of antibody to isolated glomeruli was detected by radioimmune indirect binding assay, and specificity for glomerular epithelial cells was shown using a four-layer peroxidase-antiperoxidase technique applied to epoxy resin sections of the human kidney cortex. PHM 5 appears to detect a human-specific cell surface carbohydrate antigen not previously described, and can be used to identify epithelial cells in renal biopsy sections and in culture outgrowths of isolated glomeruli.
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