Appearance of free histones within nuclei of differentiating cells in the developing rat embryo
作者:
G. Csaba,
Andrea Bukta,
P. Kovács,
期刊:
Cells Tissues Organs
(Karger Available online 1977)
卷期:
Volume 98,
issue 2
页码: 154-161
ISSN:1422-6405
年代: 1977
DOI:10.1159/000144790
出版商: S. Karger AG
关键词: Histories;Control of development;Differentiation;Rat;Erythrocytes;Histo-chemistry
数据来源: Karger
摘要:
Having applied the picric acid-bromphenol blue (BPB) and the phosphomolybdic acid-benzidine (PMA-B) methods – the first method detecting the total histone content, the second one only free histones – we have found that cell nuclei of the rat embryos permanently contain histones, nevertheless, in such a pattern that the free histone appears only at a well-defined stage of the embryogenesis. Strong PMA-B reaction is observable in the nuclei of some mesenchymal cells and in the reorganizing somites’ cell nuclei of the 13-day-old embryo as well as in the hemopoietic elements of the 15- and 17-day old embryos’ liver. Within nuclei of the red blood cells of the 13-day-old embryo an extremely intense reaction is seen which then disappears till the 15th day, while the BPB reaction shows a gradual intensification up to the neonatal age. The experiments support the former model of one of the authors for a histone-nonhistone control of onto
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