The water sorption isotherms of the cefotaxime sodium salt were determined at 30 and 40° C. Cefotaxime sodium salt was maintained at equilibrium relative humidities ranging from 40 to 80 %. Equilibrium moisture content was determined by the Karl Fischer method. The moisture sorption isotherms showed that the equilibrium moisture decrease while the temperature increase. The experimental curves of equilibrium humidity were fitted by different models (Bradley, Halsey, Henderson, Kuhn, Smith and Iglesias and Chirife and GAB). Parameters of each equations were determined by non-linear regression analysis. The best fit was obtained by Iglesias and Chirife model. The isosteric heat of moisture sorption calculated by Claussius Clapeyron equation varied from 64·45 to 48·03 kJ/mol when moisture content changed from 7 to 9·5 %.