Age Effects on Colony-Forming Human Peripheral Blood T and B Cells
作者:
Marguerite M.B. Kay,
期刊:
Gerontology
(Karger Available online 1985)
卷期:
Volume 31,
issue 4
页码: 278-284
ISSN:0304-324X
年代: 1985
DOI:10.1159/000212710
出版商: S. Karger AG
关键词: Phytohemagglutinin;Proliferation;Anti-Fab antibodies
数据来源: Karger
摘要:
An assay was developed for studying the effect of age on human colony-forming T and B cells. Phytohemagglutinin was used to induce the formation of T cell colonies and anti-human Fab antibodies were used to induce the formation of B cell colonies. The assay requires a short ( < 8 h) incubation in liquid media in the presence of a mitogen prior to plating in semisoft agar. Neither autologous serum, red cells, nor conditioned media are required to support colony formation. The kinetic and morphologic characteristics of T and B cell colonies were different. Formation of T cell macro- but not microcolonies was significantly impaired during aging. Formation of B cell colonies was decreased with aging, but the decrease was not significant.
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