A Novel Reverse Phase HPLC Method to Separate and Quantify Androstenedione and Its Stereospecific Hydroxyaromatic Derivatives
作者:
Elena Valdés,
Malaquias Vargas,
Jerson Mancilla,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 9
页码: 1867-1880
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017436
出版商: Taylor & Francis Group
关键词: Androstenedione;Microsomal Androstenedione Metabolites Separation
数据来源: Taylor
摘要:
Reverse - phase High Pressure Liquid Chromatography with a gradient elution on a LiChrocart 250-4 LiChrospher 100 RP-18 column has been used to separate and quantify 7 α-hydroxyandrostenedione (7α-OHA), 6β-hydroxyandrostenedione (6β- OHA), 16α-hydroxyandrostenedione (16α - OHA), 2 β - hydroxyandrostenedione (2 β - OHA), testosterone (T) and androstenedione (A). These steroids are the major products of androstenedione hydroxylation by adult rat liver microsomes. Separation was achieved in 30 minutes by using a linear gradient of acetonitrile (CH3CN) and water in increasing amounts from 30% to 60% of the first solvent (CH3CN).
点击下载:
PDF (316KB)
返 回