Volume-Regulatory Potassium Release from Isolated Perfused Rat Kidney
作者:
Michael Joannidis,
Harold Völkl,
Walter Pfaller,
Florian Lang,
期刊:
Kidney and Blood Pressure Research
(Karger Available online 1989)
卷期:
Volume 12,
issue 5-6
页码: 338-346
ISSN:1420-4096
年代: 1989
DOI:10.1159/000173211
出版商: S. Karger AG
关键词: Cell volume regulation;Potassium conductance;Isolated perfused proximal renal tubule;Isolated perfused rat kidney;Verapamil;Barium
数据来源: Karger
摘要:
The present study has been performed to test for cell volume regulatory potassium release from the isolated perfused rat kidney exposed to hypotonic perfusate and for its sensitivity to potassium channel blocker barium and calcium channel blocker verapamil. Replacement of 25 mmol/l NaCl with 50 mmol/l mannitol has little effect on effluent potassium activity, whereas subsequent omission of mannitol from the perfusate leads to a transient increase of effluent potassium activity, reflecting volume regulatory potassium release. Barium (1 mmol/l) leads to a marked transient decrease of effluent potassium activity, pointing to net cellular uptake of potassium. Verapamil (1 µmol/l) leads to a slight decrease of effluent potassium activity. Both barium and verapamil virtually abolish the rapid, transient increase of effluent potassium activity upon exposure to hypotonic perfusates. Thus, the substances either block or markedly retard volume regulatory potassium release. The apparent renal vascular resistance is transiently increased by exposure to hypotonic perfusates and by barium, but is reduced by verapamil. Cell volume regulation of isolated perfused mouse straight proximal tubules is retarded but not abolished by verapamil (0.1 mmol/l). In conclusion, cellular potassium release from rat kidney can be determined by continuous measurement of effluent potassium activity. The volume regulatory potassium release and cell volume regulation are impaired by both barium and verapamil. The persisting cell volume regulation could be due either to slow potassium release and/or some mechanism independent of potassium
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