Involvement of Loop 2 of Platelet-Derived Growth Faetor-AA and-BB in Receptor Binding
作者:
AnderssonMaria,
ÖstmanArne,
KreysingJoachim,
BäckströmGudrun,
van de PollMonique,
HenrikCarl,
期刊:
Growth Factors
(Taylor Available online 1995)
卷期:
Volume 12,
issue 2
页码: 159-164
ISSN:0897-7194
年代: 1995
DOI:10.3109/08977199509028962
出版商: Taylor&Francis
关键词: platelet-derived growth factor;receptor binding;mutagenesis
数据来源: Taylor
摘要:
AbstractPlatelet-derived growth factor (PDGF) is a disulfide-bonded antiparallel dimer of A-and B-polypeptide chains. Each subunit contains two loops (loops 1 and 3) which point in the same direction, and which are located close to a region (loop 2) from the other subunit of the dimer. Previous studies have shown that epitopes in loops 1 and 3 are important for binding to PDGFα-andβ-receptors. The aim of the present investigation was to determine the importance of loop 2 for receptor interactions. PDGF A-and B-chain cDNA:s were mutated in the loop 2 regions and transfected into COS cells. Analyses of conditioned media of such cell cultures revealed that PDGF B-chain mutated in the loop 2 region lost its ability to compete with125I-PDGF for binding to PDGFβ-receptors, but retained 2–5% of its binding ofα-receptors. The A-chain binds only toα-receptors; 2–5% of this binding was also retained after mutation of the loop 2 region. In conclusion, the loop 2 region of PDGF is important for receptor binding, but appears to be more important for binding to the PDGFβ-receptors than to theα-receptors.
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