Adrenergic stimulation of bovine non-pigmented ciliary epithelial cells raises cAMP but has no effect on K+or Cl-currents
作者:
GoochA. J.,
MorganJ.,
JacobT. J. C.,
期刊:
Current Eye Research
(Taylor Available online 1992)
卷期:
Volume 11,
issue 10
页码: 1019-1029
ISSN:0271-3683
年代: 1992
DOI:10.3109/02713689209033500
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Freshly isolated bovine non-pigmented ciliary epithelial cells were studied using the whole-cell voltage-clamp and permeabilized patch techniques. In a study of 148 cells three classes of cell were found; those containing inward currents alone, 31%; those containing outward currents alone, 37% and those containing both inward and outward currents, 32%.The outward currents exhibited slow, delayed activation, were blocked by tetraethylammonium (TEA+) but were not effected by changes in [Cai] suggesting they are K+-currents similar to IK(V), the delayed rectifier. The inward currents were reduced by TEA+and blocked by Cs+suggesting they are inward rectifier K+-currents.Intracellular cAMP levels were increased by isoprenaline with a Kaof 20nM. However, the resting membrane potential recorded from whole ciliary processes in intracellular microeletrode studies was not effected by adrenergic stimulation, neither were the leak current, the outward current nor the sustained inward current significantly effected by isoprenaline, noradrenaline or dibutyryl-cAMP in whole-cell and permeabilized patch clamp studies.
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